Technical Assistant (f/m/d) in T Cell Engineering and Molecular Biology Department of Neurology
Uniklinik Mannheim Stellenangebote →Liegen Ihnen Medizin und Forschung im Blut? Dann suchen wir Sie!
Die Universitätsmedizin Mannheim steht für qualitätsgesicherte, universitäre Maximalversorgung und zählt zu den größten medizinischen Einrichtungen und Forschungszentren der Region. Mit der Medizinischen Fakultät Mannheim der traditionsreichen Universität Heidelberg und dem Universitätsklinikum Mannheim vereinen wir zwei starke Partner. Jährlich bieten wir rund 52.000 stationär und teilstationär sowie 212.000 ambulant aufgenommenen Patienten ein breit gefächertes Spektrum modernster Diagnostik und Therapieverfahren. In 30 Fachkliniken und Instituten engagieren sich 5.000 Mitarbeiter gleichermaßen in Krankenversorgung, Forschung und Lehre.
The Department of Neurology (Director: Prof. Dr. Platten) at Mannheim University Medical Center is seeking to strengthen the team, as soon as possible, for a limited period of 2 years, a full-time
Technical Assistant (f/m/d) in T Cell Engineering and Molecular Biology
The Neuroimmunology and Brain Tumor Immunology (Head: Professor Michael Platten) develops personalised T cell therapies for solid tumours. Our work combines the identification and validation of tumour-reactive T cell receptors, high-throughput molecular cloning and vector engineering, and functional testing of engineered T cells in cellular model systems. We translate these findings into clinically applicable therapeutic candidates together with our clinical and industry partners.
The position is based in Heidelberg and is initially limited to 2 years.
Your tasks:
- Isolation of PBMCs from whole blood; activation, REP expansion, and cryopreservation of primary human T cells & Jurkats
- Transfection and electroporation of primary cells; production and titration of lentiviral/retroviral vectors; cell line transduction
- Performing multi-step PCR protocols to generate sequencing libraries for NGS (Illumina or ONT), basic bioinformatics (python)
- 4+ colour flow cytometry (FACS), sorting target cells within GenTG S1 and S2 biosafety requirements, DNA extraction from cells
- Laboratory management: ordering consumables, onboarding colleagues, coordinating repairs, annual inventory, etc.
Your profile:
- Completed training as a technical assistant (BTA/MTA/CTA), or degree in suitable life science subject (BSc or MSc)
- Previous experience in immunology e.g. PBMC isolation, Jurkat cell culture, experience with primary cell lines/tumors
- Experience with 4+ colour flow cytometry and with preparing DNA from sorted cells
- Experience with complex, multi-step molecular biology protocols (NGS library preparation, multi step cloning)
- Precision, commitment, teamwork, flexibility regarding tasks and approaches, organizational skills, enthusiasm
Your benefits:
- A job with a high level of responsibility and a varied range of tasks
- An open-minded and motivated team
- Offers to support the compatibility of family and career
- Support for your personal and professional development through Heidelberg University's internal educational program
- Salary in accordance with the provisions of the Collective Agreement for the Public Service of the Federal States (TV- L 9)
- Pension scheme for the public service (VBL)
We stand for equal opportunities. Severely disabled persons are given priority in the case of equal suitability.
As part of our efforts to offer a sufficient number of part-time positions, the advertised position may, in principle, be filled by two part-time employees.
For further information, please contact Vanessa Schwenk, phone 0621/383-2885).
If you have any technical questions, please contact [email protected], phone 0621/383-2885.
Application deadline: 31.10.2026 ; Reference: 811-143